Dried melon seeds (Citrullus colocynthis L.) of the family Cucurbitaceae were investigated for nutritional quality and the oil seed characteristics. These melon seeds, on a dry weight basis, consisted of 52.3% of test and 47.7% of kernel. The moisture content in melon seeds was 54.5% and the mineral constituents were also determined. The oil content of seeds was very high ranging from 22.1-53.5%, due to the presence of the hulls, 22% from the seeds and 53% of the kernel, and also the crude protein content was so high as the 21.8% of the seeds. Standard procedures were applied to determine the fatty acids composition of the seed oil. The fatty acid profiles of the seed oil showed an unsaturated fatty acid content of 77.4% and the high content of 63.2% of PUFA. The predominant fatty acid was linoleic (18:2) acid in 62.2%. The presence of other fatty acids ranged in 10-14% for oleic (18:1) stearic (18:0) and palmitic (16:0) acids, respectively. Furthermore, the physical and chemical characteristics of the seed oil was also determined as iodine, acid, saponification, peroxide values and specific gravity.