Results of the laboratory study of the quality of gentian seed are analyzed in this paper. Seed originated from wild and grown plants from Zlatibor Mountain (approx. 1000 m above sea level) collected in the third decade of August 2006 and from wild and grown gentian plants around Ivanjica (approx. 1030 m above sea level) collected also in the third decade of August 2006, as well as from grown plants in the Tara Mountain (approx. 1000 m above sea level) collected during the same period. The effects of cooling (90 days at 40°C) and moistening and cooling (90 days at 40°C) on germination energy (GE) and total germination of seed (TG) were observed. The origin of the seed (if it comes from grown or wild plant) had influence on both investigated traits, and grown seed had slightly better germination energy and higher total germination compared to seed of wild plants. On the location in Tara we had no seed from wild plants so no comparison was possible. The location also demonstrated significant effect on traits of ability for living, so wild seed from Zlatibor was of higher quality than the one collected in the vicinity of Ivanjica. In regard to seed from grown gentian the highest quality was determined in seed grown in Zlatibor Mountain, than Tara, and the poorest germination traits were exhibited by seed grown around Ivanjica. The variant with moistening and cooling of seed regardless of the location and origin possessed better germination energy and total germination compared to seed that has only been cooled, and especially in regard to the control (no cooling).