In the present work the effect of natural biostimulators and different doses of slow disintegrating fertilizer on the quality of rosemary seedlings was studied. Rosemary seedlings were produced in containers, according to the 'speedling system'. During the production of seedlings natural biostimulators Megafol and Viva and microbiological fertilizer Slavol were added. The applied biostimulators made a significant effect on the quality of rosemary seedlings. Different doses of the slow disintegrating fertilizer Scotts (Osmocote Extact) were applied (0, 1, 2, 3 and 4 g/l), which also produced a significant influence.