This paper presents a study of the profile distribution of available micronutrients Fe, Mn, Cu and Zn and radionuclide¹³⁷Cs in cultivated soil at the experimental field “Radmilovac” (property of Agricultural Faculty, Belgrade University) in the vicinity of Vinča Institute of Nuclear Sciences. The soil belongs to the anthrosol class of anthropogenic soils according to FAO (2006). At first, the deep plowing was performed while preparing soil for planting peach trees followed by cultivation of soil for 12 years. All agricultural treatments at the experimental field ceasedfor three years. After that period, soil sampling was carriedout. Contents of DTPA-extracted Fe, Mn, Cu and Zn were in the range of (mg kg^-1): 5.8–41.6; 9.2–34.2; 1–7.6 and 0.2–1.3, respectively. Detected activity concentration (Bq kg^-1) for ¹³⁷Cs ranged from 1.8 to 35. It was noticed that distribution patterns of ¹³⁷Csradionuclideand available Cu and Znalong soil depthwere very similar and they were analyzed by simple linear regression; mutual affinity for the soilorganic matter might affect theirdistribution in soil. Contents of available Fe and Mn exhibited different, more constantdistribution within a soil horizon.