The studyof the hemoglobin polymorphism was performed on blood samples, taken from areas inhabited by Pramenka sheep in Central Bosnia: the area of the mountain Vlašić, the upperflow of the riverVrbas and Kupres plateau. Dupska pramenka sheep inhabits the mountain Vlašićand the areas of surrounding municipalities. Privorska pramenka sheep inhabits the area around the upperflow of the riverVrbas and the mountain Vranica and Kupreška pramenka sheep inhabits Kupres plateau. HbA gene frequency was 0.31 and HbB gene frequency was 0.69 for Dupska pramenka sheep. Privorska pramenka sheep had a frequency of 0.33 for the HbA gene, and 0.67 for the HbB gene, in its population. Kupreška pramenka sheep had the lowest frequency of the HbA gene, (0.30), and therefore the maximum frequency of the HbB gene. Hemoglobin genotype frequencies for all investigated types of Pramenka sheep breed are in equilibrium. Investigated types of Pramenka sheep breed inhabit areas adjacent to each other.  They are phenotypically quite similar and have a similar frequency of genotype polymorphism of hemoglobin. The difference between them is not statistically significant.