This work investigates different extraction techniques by utilizing 95% ethanol and refined sunflower oil as well as the effects of thermal pretreatments on the extraction of antioxidants from oak acorns. Experiments were conducted using oak acorn from Quercus cerris and Quercus robur. Peroxide values measurements and TBA test were used for oxidation rate monitoring. Two types of extracts were prepared: Using dried acorns Using heat-treated acorns - 15 min 210°C. The results indicate that both oil and ethanol extracts have antioxidative properties. Heat treatment did not have adverse effect on antioxidative properties of oak acorn extract.