The witch-weeds (Striga spp.) are destructive root parasites of many crops. They result in considerable crop damage, especially in the semiarid tropic parts of the world. The parasite control is difficult due to the complexity of the parasitelife cycle, and the large number of seeds produced by the parasite with prolonged viability. The most promising way of controlling the parasite is through the development of the resistant crop varieties. Identification of different sources of resistance will enhance breeding for resistant varieties. Wild relatives of sorghum are rich in genetic diversity and have a broad genetic base including novel and valuable traits like Striga and disease resistance. In this context, 55 wild sorghum lines were collected from three regions of Sudan including eastern Sudan (Gadaref), central Sudan (Gezira), and western Sudan (North Kordofan). The collected germplasm was assessed for Striga resistance using artificial infestation. The results showed a significant difference in the number of Striga emerged plants compared to the checks. Hence, the significant difference was observed in the number of days to 50% plants to reach flowering, plant height, and grain yield per hectare. The wild relatives were also morphologically characterized and the result showed 55 lines structured in six groups independently from their geographical regions.