This study aims to determine the percentage of pollination of a combination of cross-pollination and self-pollination in several melon inbred lines and to determine the yield character of the combination of cross-pollination and self-pollination in F3 melon inbred lines. The study was conducted in 2021 and 2022 by using nine melon inbred lines as planting material, consisting of 4 female parents (ACL211390, ACL221402, ACL221326, and ACL231312) and 5 male parents (ACD211303, ACD211254, ACD221362, ACD231380, and ACL21402). In general, the percentage of successful pollination showed various values, and the value of 100% was not obtained from all sample plants. This was because the pollination of 3 hermaphrodite flowers (female parents) on each sample plant was carried out at different times. The results of the observations of yield characteristics (fruit weight, fruit diameter, fruit length, flesh thickness, and fruit total soluble solids) showed different values between the pollination combinations in the same female parent inbred. The differences in pollen sources were responsible for the differences in yield characteristics between the pollinated combinations with the same female parent. The Student’s t-test between the inbreds ACL211390, ACL221402, ACL221326, and ACL231312 (female parent) showed that there was no significant difference in the mean percentage of pollination success and that there were significant to very significant differences in several yield characters. The difference in yield characters was due to differences in the composition of genetic material between the inbreds ACL211390, ACL221402, ACL221326, and ACL231312.