The seed oil from a local variety Rubus idaeus L. (family Rosaceae) was isolated and the physical and chemical characteristics were determined. The oil content of Rubus idaeus seeds ranged from 8-15%. Standard procedures were applied to determine specific gravity, refractive index, acid value, saponification value, iodine value and peroxide value. The fatty acid composition was identified by GL chromatography and the main components were as follows: 9.9% oleic, 59.2% linoleic and 26.4% α-linoleic acid (ALA). Furthermore, Rubus idaeus tocopherol composition (mg/100g) was identified by HPLC, as: α-tocopherol 154.0, γ-tocopherol 192.0 and β-tocopherol 12.0. The seed oil contained 0.24 mg/L of carotene, 1.12 mg/L of chlorophyll a and 0.63 mg/L of chlorophyll b.