The enrichment of eggs with polyunsaturated fatty acids (PUFA), particularly with omega-3 fatty acids, has attracted the attention of both researchers and the food industry, because these fatty acids are essential for normal body development and play an important role in the prevention of heart diseases. This experiment was conducted to evaluate the effect of four dietary oils on selected blood metabolites in egg-type chickens. One hundred and five (105) Isa Brown laying hens at 34 weeks of age were used and the study lasted for 6 weeks in a completely randomised design. Hens were randomly allotted to seven dietary treatments namely: basal diet (T₁), basal diet + 1.5% palm oil (T₂), basal diet + 1.5% soybean oil (T₃), basal diet + 1.5% sesame seed oil (T₄), basal diet + 1.5% fish oil (T₅), basal diet + 0.75% soybean oil + 0.75% fish oil (T₆) and basal diet + 0.75% sesame seed oil + 0.75% fish oil (T₇). The treatments had 5 replicates of 3 hens each. Blood samples (5mls) were collected from the jugular vein of a bird per replicate for serum and haematological parameters. There were no significant differences observed in packed cell volume, haemoglobin, red blood cell, white blood cell, lymphocyte, heterophils and basophils of birds on experimental diets but diets had an influence (P<0.05) on the monocytes, eosinophils and platelets of birds. Monocytes of birds on the control diet were similar to those of birds on T₃ (basal diet + 1.5% soyabean oil) but significantly (P<0.05) higher than monocyte counts for birds on other diets. Eosinophils of birds on the experimental diets were similar except for those on T₄ (basal diet + 1.5% sesame seed oil) with significantly higher eosinophil values compared with others. A similar trend was observed in platelets of birds on the experimental diets.There were no significant differences observed in triglycerides, high density lipoproteins and cholesterol of birds on experimental diets. Low density lipoprotein (LDL) of birds on the control diet was similar to those recorded for birds on different dietary oils supplemented diets except for those on T₆ (basal diet + 0.75% soybean oil + 0.75% fish oil) with significantly (P<0.05) reduced LDL. It can be concluded that dietary oils combination of 0.75% soybean oil + 0.75% fish oil could be effective in reducing serum low density lipoprotein in laying birds.