Our research has been made on brown forest soil that is used in long-term experiments. The soil we used had a certain crop rotation (wheat-corn) and had been fertilized according to a certain system for 30 years. In the experiment, quantities of nitrogen fertilizers were gradually increased after which samples were taken from 0 to 30 cm depths in order to establish plant and soil parameters for assessing the applied methods. Two experiments were made: one in the field and one in a controlled environment.