It is possible to decompose starch into monosaccharides by the method of acid starch hydrolysis. By applying appropriate chemical procedure, a main solution is obtained, from which aliquots are taken after filtration to determine of the present glucose according to Luff-Shoorlu method. The analysed potatoes of cv. Desiree, grown on various sites in Serbia, have starch content that corresponds to available literature data. The highest starch content is found in potatoes from Ivanjica and Novi Pazar, i.e.